| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MENIZ, Samuel | Harvest Academy | 2:32.09 | Royal Palm Beach Invitational 2026 |
| 2 | DIXON, Christian | Clewiston |
2:34.87 | Jensen Beach Invitational 2026 |
| 3 | HERNANDEZ, Isaias | DeSoto County |
2:38.15 | Lake Placid Invitational 2026 |
| 4 | BRAVO, Franklin | Harvest Academy | 2:45.72 | Lake Placid Invitational 2026 |
| 5 | ARTEAGA, Josiah | LaBelle |
2:47.21 | 2026 South Fort Myers Wolfpack Invitational |
| 6 | GUTIERREZ-BELTRAN, Josmar | DeSoto County |
2:47.69 | Lake Placid Invitational 2026 |
| 7 | QUIROZ, Sir | LaBelle |
2:51.56 | Charlotte Invitational |
| 8 | HUMBERT, Samuel | Clewiston |
2:57.05 | Avon Park Red Devil Invitational |
| 9 | LEITNER, Aaron | Harvest Academy | 3:00.28 | Avon Park Red Devil Invitational |
| 10 | MULLINGS, Nicodemus | Clewiston |
3:02.58 | Benjamin Meet #2 2026 (Approved Qualifier) |
| 11 | MARSHALL, Jaiden | DeSoto County |
3:03.50 | Lake Placid Invitational 2026 |
| 12 | BUSSOTT, Michael | Clewiston |
NT | |
| 13 | SAINZ, Pedro | Clewiston |
NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ALFONSO, Jendris | Moore Haven |
2:11.75 | Jensen Beach Invitational 2026 |
| 2 | BARRERA, Jimmy | DeSoto County |
2:13.05 | Larry Richardson Memorial Invite |
| 3 | LEON, Valentin | Lake Placid |
2:19.24 | Avon Park Chet Brojek Track Invitational |
| 4 | HERNANDEZ-YESCA, Oscar | LaBelle |
2:19.62 | Lake Placid Invitational 2026 |
| 5 | ZEPEDA, Daniel | Lake Placid |
2:20.46 | Avon Park Chet Brojek Track Invitational |
| 6 | MELGAR, Vincent | Lake Placid |
2:25.24 | Avon Park Invitational |
| 7 | NAVARRO , Tony | Moore Haven |
2:27.32 | Avon Park Red Devil Invitational |
| 8 | GIVENS, Antonio | Clewiston |
2:28.83 | Jensen Beach Invitational 2026 |
| 9 | KIESLING, Kyler | DeSoto County |
2:29.09 | Lake Placid Invitational 2026 |
| 10 | RICO, Angel | Moore Haven |
2:30.49 | Lake Placid Invitational 2026 |
| 11 | MALDONADO, Noah | LaBelle |
2:30.80 | Avon Park Chet Brojek Track Invitational |
| 12 | RICO, Neri | Moore Haven |
2:31.98 | Lake Placid Invitational 2026 |