| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | 
|---|---|---|---|---|---|---|---|---|
| 1 | South Fork | A | Spatara | Mclaughlin | Schiff | Brown | 9:34.77 | FHSAA Class 3A Track & Field State Championships | 
| 2 | American Heritage (DB) | A | Fleurigene | Edelstein | Strickler | Fedele | 9:52.01 | FHSAA Class 2A Track & Field State Championships | 
| 3 | South Florida HEAT | A | Benson | Dorn | Dorn | Diez | 10:13.80 | FHSAA 1A Region 4 | 
| 4 | Lincoln Park | A | Drawdy | Drawdy | Lane | Duffy | 10:18.53 | FHSAA 2A Region 2 | 
| 5 | Jensen Beach | A | Haley | Gozdz | Engle | Vancour | 10:51.18 | FHSAA 3A Region 4 | 
| 6 | Sebastian River | A | Gibbs | Vecchione | Kish | Sadley | 11:07.18 | FHSAA 3A Region 4 | 
| 7 | Treasure Coast | A | Swann | Moore | Jean | Spang | 11:30.08 | FHSAA 4A District 10 | 
| 8 | Fort Pierce Central | A | Brianvil | Harrold | Moore | Walker | 12:20.59 | St. Lucie County Championships | 
| 9 | Centennial | A | Garcia-Romero | Lundgren | Dinzey | Brown | 13:54.57 | Cobra Relays 2023 |