| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | 
|---|---|---|---|---|---|---|---|---|
| 3 | American Heritage (DB) | A | St Julien | Seraphin | Rocha Carlos | Edelstein | 3:44.69 | Jensen Beach Falcon Invitational | 
| 4 | Port St. Lucie | A | Ngafeeson | Simms | Flores | Morman | 3:39.89 | FHSAA 3A District 13 | 
| 5 | South Florida HEAT | A | Dorn | Webb | Waters | Porter | 3:43.55 | FHSAA 1A District 15 | 
| 6 | Jensen Beach | A | NT | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | 
|---|---|---|---|---|---|---|---|---|
| 1 | Dwyer | A | Baptiste | Hollice | Sainvil | Griffin | 3:36.97 | Panther Relays 2023 | 
| 2 | Lincoln Park | A | Sainnatus | Rivera | Thomas | Coto | DNS | FHSAA 2A Region 2 | 
| 3 | South Fork | A | Mussenden | Kornilov | Critoph | Johnson | DNS | Treasure Lake Conference | 
| 4 | Fort Pierce Central | A | Beauford | Mitchell | Thompson | Kaegi | 3:28.21 | FHSAA 4A Region 3 | 
| 5 | Martin County | A | Swindler | Hochstein | Zucker | Rodriguez | 3:31.82 | FHSAA 4A District 10 | 
| 6 | Sebastian River | A | Campbell | Perez | Johnson | McMann | 3:33.42 | 2022 Holy Trinity Invitational | 
| 7 | Fort Pierce Westwood | A | celisca | Wheeler | Scucess | Rosiles | 3:35.98 | St. Lucie County Meet 2023 | 
| 8 | Centennial | A | Bacchas | Baez | Glymph | Sterling | 3:37.24 | Jensen Beach Falcon Invitational |