| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | 
|---|---|---|---|---|---|---|---|---|
| 3 | Port St. Lucie | A | McDonald | Torres | Johnson | Brown | 4:41.71 | Treasure Lake Conference | 
| 4 | Pine School | A | Escobedo | Galbraith | Stringfellow | DeGeorge | DNS | FHSAA 1A Region 4 | 
| 5 | Treasure Coast | A | Fernandez | Braziel | Anthonia | Hatcher | 4:36.08 | Cobra Relays 2023 | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | 
|---|---|---|---|---|---|---|---|---|
| 1 | Martin County | A | Manning | Brown | Proctor | Hochstetter | 4:20.39 | Martin County Crossing the Finish Line Invite | 
| 2 | South Florida HEAT | A | Benson | Diez | Fee | Diez | 4:12.88 | FHSAA 1A Region 4 | 
| 3 | South Fork | A | Mclaughlin | Spatara | Crouse | Podbeusek | 4:11.75 | Martin County Crossing the Finish Line Invite | 
| 4 | American Heritage (DB) | A | Watts | Edelstein | Fedele | De Gruttola Aere | 4:06.57 | FHSAA 2A Region 4 | 
| 5 | Fort Pierce Central | A | Lincifort | Randolph | Domashinski | Nathan-Marsh | 4:09.09 | Cobra Relays 2023 | 
| 6 | Lincoln Park | A | Daniel | Brown | Smith | Watson | 4:12.71 | FHSAA Class 2A Track & Field State Championships | 
| 7 | Sebastian River | A | Narcisse | Alexis | Adams | Vecchione | 4:16.36 | Cobra Relays 2023 | 
| 8 | Centennial | A | Wright | Williams | Gonzalez-Trejo | Dorelus | 4:21.16 | Cobra Relays 2023 |