| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ENSOR, Emma | South Fork |
12:36.00 | FHSAA 3A District 14 |
| 2 | SNYDER, Tess | Martin County |
13:45.21 | John I Leonard Invitational |
| 3 | JEAN, Sumarah | Centennial |
18:21.57 | Viera High School Invitational |
| 4 | CANTU, Sophia | Martin County |
NT |