| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | South Fork |
A | Beard | Bonilla | Venuti | Mussenden | 46.20 | Bulldog Invitational 2025 |
| 4 | Martin County |
B | Dennis | Kideys | Gilbride | Kearns | DNS | Cobra Relays 2025 |
| 5 | Martin County |
A | Dixon | Fucile | Smith | Lewis | 44.96 | Cobra Relays 2025 |