| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Martin County |
A | Orellana | Lopes | Crescenzo | Reese | 8:36.80 | Cobra Relays 2025 |
| 2 | South Fork |
A | Nogueira | Stoiber | Hartley | Christoff | 9:26.40 | Cobra Relays 2025 |
| 3 | South Fork |
B | Patten | Adams | Harris | Rebholz | 9:26.40 | Cobra Relays 2025 |