| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MOORE, Kaitlynn | South Fork |
12:22.34 | Bulldog Invitational 2025 |
| 2 | ENSOR, Emma | South Fork |
12:28.94 | Bulldog Invitational 2025 |
| 3 | RAPAPORT, Elisabeth | Martin County |
13:22.04 | John I Leonard Invitational |
| 4 | CANTU, Sophia | Martin County |
13:43.60 | Jupiter Warrior Invitational 2025 |
| 5 | SNYDER, Tess | Martin County |
13:45.21 | John I Leonard Invitational |
| 6 | SCHIFF, Sydney | South Fork |
NT | NT |
| 7 | STRINGFELLOW, Fripp | Pine School |
NT | NT |