| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ROGERS, Jacob | South Fork |
NT | NT |
| 2 | QUINTERO, Jayden | South Fork |
1:01.08 | Dr Garcia Invite #1 2025 |
| 3 | INEUS, Leo | Martin County |
55.35 | Dr Garcia Invite #1 2025 |
| 4 | MCLEAN, Adrian | Martin County |
53.50 | Benjamin Invite #1 2025 (Sanctioned) |
| 5 | ST. JUSTE, David | Martin County |
55.02 | Benjamin Invite #1 2025 (Sanctioned) |
| 6 | ALLISON, Daniel | Martin County |
56.04 | Jupiter Warrior Invitational 2025 |
| 7 | LEIGHTY, Zach | Pine School |
NT | NT |
| 8 | REBHOLZ, Riley | South Fork |
NT | NT |