| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | HOROWITZ, Adela | Pine School |
NT | NT |
| 4 | PEPSIDERO, Alexandria | South Fork |
30.41 | Bulldog Invitational 2025 |
| 5 | PADGETT, Abigayle | South Fork |
31.07 | Bulldog Invitational 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | WEICK, Keilani | South Fork |
28.53 | Dr Garcia Invite #1 2025 |
| 3 | PEREZ, Ariana | Martin County |
28.27 | Benjamin Invite #1 2025 (Sanctioned) |
| 4 | GARCIA, Sophia | Martin County |
27.48 | Bulldog Invitational 2025 |
| 5 | HOLMES, Ava | South Fork |
27.95 | Bulldog Invitational 2025 |
| 6 | EBANKS, Angelique | Martin County |
28.28 | MCHS Fesh/Soph 2025 |
| 7 | FERNANDES, Esther | Martin County |
29.46 | Dr Garcia Invite #1 2025 |