| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | PATTEN, John | South Fork |
NT | NT |
| 3 | VENUTI, Liam | South Fork |
1:09.54 | Bulldog Invitational 2025 |
| 4 | WHITE, Luke | Martin County |
1:02.82 | Benjamin Invite #1 2025 (Sanctioned) |
| 5 | RIVERA, Ricardo | Martin County |
1:06.81 | Cobra Relays 2025 |
| 6 | BILODEAU, Jett | South Fork |
1:25.10 | MCHS Fesh/Soph 2025 |