| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Jensen Beach |
A | Huston | Sullivan | Chrysler | Huston | 3:51.00 | Jensen Beach Meet #2 |
| 4 | South Fork |
A | Zeller | Venuti | Parker-Roach | Rogers | 3:29.86 | Kings Academy Varsity 2 Sanctioned Meet |
| 5 | Martin County |
A | Dennis | St. Juste | Orellana | Ziskinder | 3:38.14 | Sarge Memorial Hosted by MCHS 2026 |
| 6 | Pine School |
A | Anderson | Devaney | Bush | Leighty | 4:03.45 | Benjamin Invite #1 2026 (approved Qualifier) |