| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | EKSTROM, Tilde | Pine School |
22.10 | MCHS Tiger Invite 2024 |
| 3 | PODBEUSEK, Alleigh | South Fork |
19.16 | South Fork Meet #1 2024 |
| 4 | SZPREGLEWSKI, Julia | Martin County |
18.00 | MCHS Sarge Invite 2024 |
| 5 | WASH, Heather | South Fork |
18.79 | MCHS Tiger Relays |
| 6 | WILLIAMS, Aubrey | Pine School |
21.88 | South Fork Meet #1 2024 |