| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SARACINA, Canyon | Martin County |
5:25.98 | Jensen Beach / Chandler Wealth Invitational 2024 |
| 2 | CRESCENZO, Matthew | Martin County |
4:57.57 | Martin County Sarge Invitational 2023 |
| 3 | DIOQUINO, Sean | Martin County |
5:14.03 | Martin County Championships 2024 |
| 4 | BORLAUG, Anthony | Martin County |
5:13.73 | MCHS Fresh / Soph 2024 |
| 5 | SLATE, Ayden | Martin County |
NT |