| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | STOVER, Livia | Martin County |
NT | |
| 3 | GARCIA, Sophia | Martin County |
28.42 | 28.42 MCHS Fresh / Soph 2024 |
| 4 | ANTENOR, C'aja | Martin County |
27.05 | 27.05 Martin County Championships 2024 |
| 5 | PEREZ, Ariana | Martin County |
28.08 | FHSAA 4A District 10 |
| 6 | KUCHENBECKER, Naomi | Martin County |
NT |