| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 3 | Treasure Coast Classical | A | DNS | MCHS Fesh/Soph 2025 |
| 4 | Okeechobee |
A | DQ | Sebring Relay Meet 2025 |
| 5 | Lincoln Park |
A | DNF | SLC Championships 2025 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | John Carroll |
A | 45.29 | Benjamin Invite #1 2025 (Sanctioned) |
| 2 | Sebastian River |
A | 44.30 | 2025 Merritt Island Relays |
| 3 | Tradition Prep | A | 43.87 | Bulldog Invitational 2025 |
| 4 | Vero Beach |
A | 42.79 | Jensen Beach Invite 2025 |
| 5 | Fort Pierce Central |
A | 43.75 | Cobra Relays 2025 |
| 6 | Fort Pierce Westwood |
A | DNS | Cobra Relays 2025 |
| 7 | Martin County |
A | 44.96 | Cobra Relays 2025 |