| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Lincoln Park |
A | NT | |
| 3 | Treasure Coast Classical | A | 1:02.05 | MCHS Fesh/Soph 2025 |
| 4 | Tradition Prep | A | 54.60 | Jensen Beach Invite 2025 |
| 5 | St. Edward's |
A | DNS | FPC Fresh/Soph Meet 2025 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Fort Pierce Central |
A | 51.80 | Cobra Relays 2025 |
| 3 | Sebastian River |
A | 49.56 | Rockledge Raider Classic |
| 4 | Vero Beach |
A | 47.77 | Bill Wilson Invitational 2025 |
| 5 | Fort Pierce Westwood |
A | 49.45 | Bill Wilson Invitational 2025 |
| 6 | Martin County |
A | 51.00 | Jensen Beach Invite 2025 |
| 7 | Okeechobee |
A | 54.33 | Jensen Beach Invitational 2025 |