| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Martin County |
A | 10:26.97 | MCHS Kenna Wilson Memorial Meet 2025 |
| 2 | St. Edward's |
A | 10:46.02 | FPC Fresh/Soph Meet 2025 |
| 3 | Fort Pierce Central |
A | 11:43.18 | Cobra Relays 2025 |
| 4 | Lincoln Park |
A | 11:54.96 | MCHS Kenna Wilson Memorial Meet 2025 |
| 5 | Treasure Coast Classical | A | 12:38.35 | Benjamin Invite #2 2025 (Sanctioned) |
| 6 | Okeechobee |
A | 13:03.92 | Rockledge Raider Classic |
| 7 | Tradition Prep | A | DNS | Jensen Beach Invite 2025 |