| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Martin County |
A | 8:36.59 | MCHS Kenna Wilson Memorial Meet 2025 |
| 2 | Lincoln Park |
A | 8:54.38 | FPC Fresh/Soph Meet 2025 |
| 3 | Fort Pierce Central |
A | 8:58.90 | Cobra Relays 2025 |
| 4 | Sebastian River |
A | 8:59.70 | Bill Wilson Invitational 2025 |
| 5 | Port St. Lucie |
A | 9:18.05 | SLC Championships 2025 |
| 6 | St. Edward's |
A | 9:25.31 | Benjamin Invite #1 2025 (Sanctioned) |
| 7 | Okeechobee |
A | DNS | Sebring Meet #2 2025 |
| 8 | Tradition Prep | A | 10:06.36 | Jensen Beach Invite 2025 |
| 9 | John Carroll |
A | 10:15.86 | SLC Championships 2025 |
| 10 | Pine School |
A | 10:19.40 | Jensen Beach Invite 2025 |
| 11 | Treasure Coast Classical | A | 10:27.30 | MCHS Fesh/Soph 2025 |