| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | PLUVIOSE, Jean | Fort Pierce Central |
NT | |
| 3 | LOUISSAINT, Vital | Fort Pierce Central |
NT | |
| 4 | CAMBRAN, Jahleel | Dwyer |
NT | |
| 5 | JEAN, Chris | Fort Pierce Central |
NT | |
| 6 | PARR, Luke | Jensen Beach |
NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | HOLLICE, Johnny | Dwyer |
1:00.00 | |
| 3 | CHARLECIUS, Zachary | Fort Pierce Central |
1:00.00 | |
| 4 | WILLIAMS, Trenton | Dwyer |
57.33 | FHSAA 4A Region 2 |
| 5 | WHITMORE, Brycen | Dwyer |
59.96 | Bob Hayes High School Invitational |
| 6 | FLOWERS, Raythez | Fort Pierce Central |
1:00.00 | |
| 7 | BRADSHAW, Carson | Dwyer |
NT |