| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MERRITT, Shannon | Fort Pierce Central |
NT | |
| 2 | SCHWARTZ, Isla | Martin County |
NT | |
| 3 | LINDA, Rachel | Martin County |
6:10.00 | |
| 4 | MARTINEZ BARRIOS, Regina | Fort Pierce Central |
5:57.30 | MCHS Fesh/Soph 2025 |
| 5 | BUSSOM, Madelyn | Martin County |
5:55.00 | |
| 6 | FISCHER, Madelyn | Jensen Beach |
5:40.13 | 5:40.13 Bill Wilson Invitational 2025 |
| 7 | RAPAPORT, Elisabeth | Martin County |
5:54.03 | FHSAA 4A District 8 |
| 8 | ESSER, Margot | Martin County |
NT |