| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Jensen Beach |
A | Acheampong | Melda | Savdas | Readling | DNF | Bill Wilson Invitational 2025 |
| 3 | Martin County |
A | Stover | Rapaport | Rettig | Richkarday | DNF | Cobra Relays 2025 |
| 4 | South Fork |
A | Laird | Smith | Angell | Rodgers | DNF | FHSAA 3A District 13 |
| 5 | Lincoln Park |
A | Brown | Anderson | Sessanna | Powell | DNF | FHSAA 2A Region 2 |
| 6 | Fort Pierce Central |
A | Veal | Paul | Spence | Stinson | 4:23.48 | Cobra Relays 2025 |