| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Fort Pierce Central |
A | Stinson | Simeus | Alexander | Brown | 51.80 | Cobra Relays 2025 |
| 4 | Martin County |
A | Prusenko | Ramlagan | Meglis | Ebanks | 50.18 | FHSAA 4A Region 2 |
| 5 | Lincoln Park |
A | Huskin | Brown | Webster | Brianvil | 50.95 | Bill Wilson Invitational 2025 |
| 6 | Jensen Beach |
A | Bradley | Laurent | Melda | Peruski | 54.40 | Jensen Beach Invitational 2026 |