| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Martin County |
A | Esser | Harper | Scott | Rettig | 10:13.45 | FHSAA 4A District 8 |
| 2 | Lincoln Park |
A | Avilla | Keshtmand | Voelker | Sanchez-Appell | 11:29.31 | FHSAA 2A Region 2 |
| 3 | Fort Pierce Central |
A | Brown | Romelus | Merritt | Blue | 11:38.53 | MCHS Sarge Invitational 2025 |
| 4 | John Carroll |
A | DUFFY | Russell | ANDERSEN | CAZARES | 11:43.58 | Vero Beach HS 2026 Preseason Classic |