| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Fort Pierce Central |
A | Victorin | Lamarre | Curry | Baker | DNF | Cobra Relays 2025 |
| 4 | Centennial |
A | Salcedo | Hernandez-Oakes | Benito | Ramezani | DNF | FHSAA State Track & Field Championships (4A) |
| 5 | Martin County |
A | Oh | Ocampo | Rael | Allison | DNF | FHSAA 4A Region 2 |