| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lincoln Park |
A | Jefferson | Rosado | Wahrmann | Webb | 8:41.30 | FHSAA 2A District 7 |
| 2 | Jensen Beach |
A | Sanchez | Smart | Gebus-Mockabee | Francisco-Perez | 8:42.57 | The Lincoln Knowles Invitational 2025 |
| 3 | South Fork |
A | Anstett | Hetzel | McDermott | Szpregelewski | 9:26.40 | Cobra Relays 2025 |
| 4 | John Carroll |
A | Duffy | MAURICIO | Schildwatcher | ASHER | 9:47.20 | Vero Beach HS 2026 Preseason Classic |