| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | VIGNIER, Selah | West Shore |
5:30.00 | |
| 2 | GUTIERREZ, Amelia | West Shore |
5:55.00 | |
| 3 | MARTINEZ-MAGANA, Seleny | John Carroll |
6:07.38 | St. Lucie County Meet 2023 |
| 4 | TWEEDY, Charlotte | Melbourne |
6:41.74 | Satellite High Reggie Johnson Memorial |
| 5 | MIKA, Emma | West Shore |
7:00.00 | |
| 6 | ARMSTRONG, Reese | West Shore |
7:30.00 | |
| 7 | PETERSEN, Audrey | Melbourne |
8:21.77 | FHSAA 4A District 10 |
| 8 | CONNEEN, Sophia | Melbourne |
NT |