| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Pineapple Cove |
A | Reich | Mclin | Ahmad | Stokes | 54.35 | Kowboy Invitational (Qualifier) |
| 4 | Dreyfoos |
A | Mcdow | Vasallo | Cannon | Pierre | 51.61 | Royal Palm Beach Invitational 2026 |
| 5 | Jupiter Christian |
A | Winslow | Winslow | Kidwell | Schiralli | 53.15 | Calvary Christian FTL Varsity Meet #1 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | South Fork |
A | Barnett | Holmes | Marrone | Pepsidero | 51.58 | Kings Academy Varsity 2 Sanctioned Meet |
| 2 | Wellington |
A | Carbocci | Brooks | Richards | Lowe | DNF | 2026 PBC County Championships |
| 3 | Suncoast |
A | Louis | Johnson | Felin | Hopkins | DNS | FHSAA 3A Region 4 |
| 4 | Dwyer |
A | Jean | McClean | Verdecia Perez | Smith | 48.40 | FSU Relays (High Schools) 2026 |
| 5 | Centennial |
A | Hoskins | Jones | Schmook | Green | 48.42 | Pepsi Florida Relays |
| 6 | Palm Beach Gardens |
A | Collier | Perry | Hooks | Mcgee | 49.56 | Royal Palm Beach Invitational 2026 |
| 7 | Martin County |
A | Edwards | Fernandes | Osdon | Perez | 50.18 | FHSAA 4A Region 2 |