| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Dwyer |
A | Quince | Martinez | Bradshaw | Williams | 3:25.40 | 2026 PBC County Championships |
| 2 | Wellington |
A | Guido | Borgella | Paul | Davis | 3:24.60 | FHSAA 4A District 13 |
| 3 | Pineapple Cove |
A | Nina | Granados-Garcia | Lucas | Guerra | 3:41.70 | FHSAA 1A District 8 |
| 4 | Suncoast |
A | Menardy | Okoh | Xue | Millien | 3:35.27 | Palm Beach Gardens Invite #1 2026 |
| 5 | Martin County |
A | Dennis | Orellana | Ziskinder | Allison | 3:38.14 | Sarge Memorial Hosted by MCHS 2026 |
| 6 | Centennial |
A | Dimarco | Johns | Polynice | Lynch | 3:16.32 | Pepsi Florida Relays |
| 7 | Jupiter |
A | Musil | Diaz | Swarr | Swarr | 3:25.19 | Dr. Garcia Meet #3 2026 |
| 8 | South Fork |
A | Rogers | Venuti | Zeller | Parker-Roach | 3:29.86 | Kings Academy Varsity 2 Sanctioned Meet |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Forest Hill |
A | Rosales | White Calderon | Bragdon | Williams | DNS | Atlantic HS Invitational 2 |
| 2 | Jupiter Christian |
A | Brandon | Gilliam | Penner | Franco | DNS | FHSAA 1A Region 4 |
| 3 | Treasure Coast | A | Austin | Diejuste | Davis | Jones | DNS | FHSAA 4A Region 2 |
| 4 | Seminole Ridge |
A | Linton | Charleston | Archambault | Allen | DNS | Dr. Garcia Meet #2 |
| 5 | Cardinal Newman |
A | Gantt | Charles | Elam | Murzynski | DNS | Florida Relays |
| 6 | Palm Beach Gardens |
A | Campbell | Jean-Baptiste | McRae | Coicou | DNS | FHSAA 4A Region 2 |
| 7 | Jensen Beach |
A | Galdys | Acosta | Belville | Reamer | DNS | Martin County Championships 2026 |
| 8 | Dreyfoos |
A | Joseph | Roby | Aquel-Lacayo | Riche | DNS | St Andrews Varsity 3 |