| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Jensen Beach |
A | Correa | Feurer | Garbellano | Glover | 4:43.04 | Bill Wilson Invitational 2025 |
| 4 | Santaluces |
A | Donalds | Auguste | Bell | Casseus | 4:38.49 | Panther Relays 2025 |
| 5 | Suncoast |
A | Hester | Anim Addo | Felin | Johnson | 4:39.08 | Royal Palm Beach Invitational 2025 |
| 6 | Inlet Grove |
A | Steward | Sharpe | Harper | Leveille | DNS | Pahokee Andre Powell Invitational |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Spanish River |
A | D'Emic | Downes | Larancuent | Popescu | 4:34.94 | Spanish River Invitational 2025 |
| 2 | Oxbridge Academy |
A | Wajer | Duke | Spurlock | Wangombe | DNS | Atlantic HS Invitational 2 |
| 3 | Dwyer |
A | Blackwell | Odita | Nichols | Jean | 4:14.32 | PBC Meet 2025 |
| 4 | Park Vista |
A | Dawkins | Hernandez | Bell | Colt | 4:02.67 | Florida Relays |
| 5 | South Fork |
A | Robinson | Mclaughlin | Weick | Brinn | 4:09.37 | Martin County Championships 2025 |
| 6 | Dreyfoos |
A | Vasallo | Mcdow | Pierre | Cannon | 4:19.78 | PBC Meet 2025 |
| 7 | Seminole Ridge |
A | Davies | Allen | Alvin | Jean | DNS | Dr. Garcia Invite #2 2025 |
| 8 | Palm Beach Central |
A | Echavarria | Mata-Rodriguez | Fabinger | Tortora | 4:36.84 | Dr Garcia Invite #1 2025 |