| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Palm Beach Lakes |
A | Joseph | Ennis | Heights | Lucas | 4:44.05 |
| 2 | Atlantic Christian |
A | Sears | Santana | Hughes | Chase | 4:34.34 |
| 3 | Dreyfoos |
A | Beauchamp | Mcdow | Vasallo | Pierre | 4:22.76 |
| 4 | Dwyer |
A | Mccalla | Harper | Jean | Verdecia Perez | 4:20.17 |
| 5 | Centennial |
A | Plantin | Africian | Hoskins | Dorelus | DNS |
| 6 | Palm Beach Central |
A | Sinclair | Rejouis | Hoffman | Taylor | 4:23.92 |
| 7 | Royal Palm Beach |
A | sainvil | Fils | Neptune | Zwirner | 4:37.36 |
| 8 | SLAM (West Palm Beach) |
A | Barnett | Canty | Vargas Lopez | Sosa | 5:05.03 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Sebastian River |
A | Bradley | Wiggins | Alexis | Gibson | 4:18.38 |
| 2 | South Fork |
A | Robinson | Ragazzon | Yanora | Weick | 4:16.66 |
| 3 | Park Vista |
A | Chu | Love | Harrigan | Wrathell | 4:14.65 |
| 4 | American Heritage (DB) |
A | Strickler | Harrell | Canales | Milord | 4:05.59 |
| 5 | King's Academy |
A | McGould | Rodriguez | Storey | Sanchez | 4:14.20 |
| 6 | Wellington |
A | Shelby | Roberts | Ramirez | Brooks | 4:15.30 |
| 7 | Jupiter |
A | Norzea | Stewart | Johnson | Monterroso | 4:16.89 |
| 8 | Seminole Ridge |
A | Garced | Touma | Shakes | James | 4:19.88 |