| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | DeSoto County |
A | 57.51 | Dragons Invitational |
| 3 | LaBelle |
A | 53.83 | Dragons Invitational |
| 4 | Sebring |
A | 53.14 | Lake Placid Meet #2 |
| 5 | Lake Placid |
A | DNS | Lake Placid Meet #2 |
| 6 | Avon Park |
A | 54.61 | Lake Placid Meet #2 |