| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Sebring |
A | 8:49.06 | Clewiston Big Lake Challenge 2024 |
| 2 | Okeechobee |
A | DNS | Clewiston Big Lake Challenge 2024 |
| 3 | LaBelle |
A | 9:37.07 | Edison Relays 2024 (Approved Sanctioned Meet) |
| 4 | DeSoto County |
A | 9:43.54 | Lake Placid Meet #1 |
| 5 | Lake Placid |
A | 10:07.04 | Lake Placid Meet #1 |