| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | PEREZ-OLVERA, Alejandra | DeSoto County |
NT | NT |
| 4 | NUNEZ, Karen | Lake Placid |
NT | NT |
| 5 | TAYLOR, Tenaj | LaBelle |
NT | 1:20 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CORTES, Ana | Lake Placid |
NT | NT |
| 2 | BERNAL, Yuridia | Okeechobee |
1:22.86 | Clewiston Big Lake Challenge 2024 |
| 3 | MARTINEZ, Clara | DeSoto County |
1:22.64 | Lake Placid Meet #2 |
| 4 | VIRKLER, Elisa | Avon Park |
1:19.78 | Lake Placid Meet #1 |
| 5 | ONATE, Jazmine | Sebring |
1:22.31 | Clewiston Big Lake Challenge 2024 |
| 6 | VALDOVINOS, Lizeth | DeSoto County |
1:22.67 | Lake Placid Meet #1 |
| 7 | GONZALEZ DEL REAL, Sofia | Sebring |
1:37.88 | Dragons Invitational |
| 8 | LETHBRIDGE, Meghan | Sebring |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | THOMASON, Gabriella | Avon Park |
1:16.62 | Lake Placid Meet #2 |
| 2 | CASSEUS, Rebecca | LaBelle |
1:08.95 | Dragons Invitational |
| 3 | MADISON, Ke'Nise | Lake Placid |
1:06.02 | IMG Spring Break Invitational |
| 4 | MADISON, Ka'Naya | Lake Placid |
1:04.95 | IMG Spring Break Invitational |
| 5 | ZANDER, Madison | LaBelle |
1:05.29 | Lake Placid Meet #1 |
| 6 | CHAIREZ, Leslie | LaBelle |
1:07.86 | Dragons Invitational |
| 7 | MENDOZA, Jasmin | Sebring |
1:15.47 | Clewiston Big Lake Challenge 2024 |
| 8 | HANNON, Jananarra | DeSoto County |
1:17.87 | Lake Placid Meet #2 |