| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | REED, Jada | DeSoto County |
NT | NT |
| 2 | JARVIS, Zarionna | DeSoto County |
33.48 | Lake Placid Meet #2 |
| 3 | BERNAL, Yuridia | Okeechobee |
31.91 | Clewiston Big Lake Challenge 2024 |
| 4 | SACK, Emma | Sebring |
31.17 | Lake Placid Meet #2 |
| 5 | JOHNSON, Ariel | DeSoto County |
31.83 | Lake Placid Meet #1 |
| 6 | ORTIZ RODRIGUEZ, Isamary | Okeechobee |
32.75 | Lake Placid Meet #2 |
| 7 | MONTOYA, Tiare | LaBelle |
35.96 | Bishop Verot - Viking Elite Classic (Regional Qualifier) |
| 8 | MCMILLAN, Sereana | Sebring |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | JOHNSON, Shamera | Lake Placid |
30.26 | Lake Placid Meet #2 |
| 2 | WHEATLE, Asania | LaBelle |
29.37 | Clewiston Big Lake Challenge 2024 |
| 3 | SANDOVAL, Anakaren | LaBelle |
28.28 | Clewiston Big Lake Challenge 2024 |
| 4 | HAWTHORNE, Ramiya | Sebring |
27.55 | Lake Placid Meet #2 |
| 5 | GLABACH, Myra | Avon Park |
28.17 | Lake Placid Meet #1 |
| 6 | SANDOVAL, Valerie | LaBelle |
28.78 | Lake Placid Meet #1 |
| 7 | DILLON, Mykala | Lake Placid |
30.13 | Dragons Invitational |
| 8 | DESTINO, Zaymiah | Avon Park |
30.99 | Lake Placid Meet #2 |