| Lane | # | Athlete | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | 40 | THOMPSON, KASH | LaBelle |
NT | NT |
| 3 | 219 | AMES, HAYDON | DeSoto County |
21.53 | Lake Placid Meet #1 |
| 4 | 296 | BULLARD, JR., DOUGLAS | Lake Placid |
17.15 | IMG Spring Break Invitational |
| 5 | 131 | WILKERSON, ZAYDEN | Sebring |
21.43 | 2024 Hardee Wildcat Relay Meet |
| 6 | 249 | ZEPEDA, FERNANDO | DeSoto County |
23.24 | Lake Placid Meet #1 |