| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | VIRKLER, Elisa | Avon Park |
NT | NT |
| 2 | MISCAIDE, Estaina | Clewiston |
NT | NT |
| 3 | JOHNSON, Mahagony | Avon Park |
NT | NT |
| 4 | CAMPBELL, Lailah | Lake Placid |
1:25.06 | Avon Park Meet #1 2025 |
| 5 | DIAZ, Arlene | Lake Placid |
1:27.19 | Avon Park Meet #1 2025 |
| 6 | RAMIREZ, Michelle | Clewiston |
NT | NT |
| 7 | ZANDER, Madison | LaBelle |
NT | 1:05 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ZUNIGA, Natalie | LaBelle |
1:15.35 | Charlotte Invitational |
| 2 | GREENE, Ja'zariah | Avon Park |
1:09.56 | Avon Park Meet #1 2025 |
| 3 | JACKSON, Amari | LaBelle |
1:08.72 | Charlotte Invitational |
| 4 | GLABACH, Myra | Avon Park |
1:06.82 | Avon Park Meet #1 2025 |
| 5 | JOHNSON, Hannah | Clewiston |
1:07.83 | 65th Annual Edison Relays |
| 6 | CHAIREZ, Leslie | LaBelle |
1:08.75 | 65th Annual Edison Relays |
| 7 | CARLINI-SMITH, Ayva | Lake Placid |
1:11.39 | Avon Park Meet #1 2025 |
| 8 | SANTIAGO, Gabriela | Lake Placid |
1:23.69 | Avon Park Meet #1 2025 |