| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | GALLEGOS, Adyn | LaBelle |
NT | NT |
| 2 | SEVERO, Alexander | Avon Park |
NT | NT |
| 3 | MULLINGS, noah | Clewiston |
NT | NT |
| 4 | SIMMONS, Kalib | LaBelle |
1:02.98 | 65th Annual Edison Relays |
| 5 | MCKENZIE, kerream | Clewiston |
NT | NT |
| 6 | GUILLEN, Jose | LaBelle |
NT | NT |
| 7 | COHEN, Diangelo | Avon Park |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | WASHINGTON, Jayquemini | Lake Placid |
1:01.63 | Avon Park Meet #1 2025 |
| 2 | MULLINGS, Nehemiah | Clewiston |
58.38 | Spanish River Invitational 2025 |
| 3 | WALTON, Terrell | Lake Placid |
54.30 | Avon Park Meet #1 2025 |
| 4 | CHARITE, Kepson | Lake Placid |
53.50 | Avon Park Meet #1 2025 |
| 5 | ANDERSON, D'Ylyn-Nikym | Lake Placid |
53.88 | Avon Park Meet #1 2025 |
| 6 | WATKINS, Jason | Avon Park |
54.40 | Frostproof - The Ridge Open 2025 |
| 7 | TIMMONS, Jahari | Avon Park |
1:01.26 | Frostproof - The Ridge Open 2025 |
| 8 | WILLIAMS, Ryder | Clewiston |
1:02.55 | Kings Academy Varsity 2 |