| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | TAYLOR, Tenaj | LaBelle |
NT | 31 |
| 2 | JACKSON, Jamara | Avon Park |
NT | NT |
| 3 | HENRY, Alayah | Lake Placid |
35.60 | Avon Park Meet #1 2025 |
| 4 | CARLINI-SMITH, Ayva | Lake Placid |
30.26 | Avon Park Meet #1 2025 |
| 5 | LEGREE, Shatajah | Lake Placid |
35.05 | Avon Park Meet #1 2025 |
| 6 | EASON, Rayven | LaBelle |
NT | 32 |
| 7 | JACKSON, Amari | LaBelle |
NT | 28 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | WHEATLE, Asania | LaBelle |
29.66 | Charlotte Invitational |
| 2 | STEPHENS, Jahpheria | Clewiston |
29.35 | Kings Academy Varsity 2 |
| 3 | MCCAIN, Shania | Clewiston |
28.74 | 65th Annual Edison Relays |
| 4 | WATT, Kamila | Avon Park |
27.69 | Avon Park Meet #1 2025 |
| 5 | DILLON, Makayla | Lake Placid |
28.69 | Avon Park Meet #1 2025 |
| 6 | BRADFORD, Juli | Clewiston |
29.13 | Kings Academy Varsity 2 |
| 7 | GLABACH, Myra | Avon Park |
29.38 | Frostproof - The Ridge Open 2025 |
| 8 | DAVI, Mia | Avon Park |
30.19 | Avon Park Meet #1 2025 |