| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | SIMMONS, Kalib | LaBelle |
NT | NT |
| 4 | WILSON, Eric | Avon Park |
NT | |
| 5 | THACH, Justin | LaBelle |
NT | NT |
| 6 | GRAHAM, Jordan | Clewiston |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | WATKINS, Jason | Avon Park |
NT | NT |
| 2 | ANDERSON, D'Ylyn-Nikym | Lake Placid |
24.93 | Avon Park Meet #1 2025 |
| 3 | COHEN, Diangelo | Avon Park |
24.41 | Frostproof - The Ridge Open 2025 |
| 4 | DAVIS, Arrie | LaBelle |
24.06 | 65th Annual Edison Relays |
| 5 | JORDAN, Nasir | Avon Park |
24.41 | Frostproof - The Ridge Open 2025 |
| 6 | BRADSHAW, Marquay | LaBelle |
24.83 | Charlotte Invitational |
| 7 | RIVERA, Tyee Joshua | Lake Placid |
26.55 | Avon Park Meet #1 2025 |
| 8 | SZUCS, Izsak | Clewiston |
NT | NT |