| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BURGESS JR., Laurence | Avon Park |
1:15.98 | Avon Park Meet #1 2025 |
| 2 | KING, Da'Shaun | LaBelle |
1:15.48 | 65th Annual Edison Relays |
| 3 | GRAHAM, Jordan | Clewiston |
1:10.15 | Kings Academy Varsity 2 |
| 4 | PAYNE, Jabari | Avon Park |
1:08.52 | Avon Park Meet #1 2025 |
| 5 | ROBINSON, Brandon | Clewiston |
1:09.75 | Kings Academy Varsity 2 |
| 6 | MILLER, Ja'yln | Clewiston |
1:13.65 | Kings Academy Varsity 2 |
| 7 | MAGANA, Sean | Clewiston |
1:15.74 | Spanish River Invitational 2025 |