| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | PADRON, Anahi | DeSoto County |
25.85 | Dunbar Tiger Classic Invite (Approved Qualifier) |
| 4 | SANTIAGO, Sophia | Lake Placid |
22.35 | Avon Park Red Devil Invitational |
| 5 | SEVERE, Erianna | Hardee |
23.44 | Pirate Booty Invitational- Approved Qualifier |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | WHITE, Sa'Niyah | DeSoto County |
21.29 | Avon Park Invitational |
| 3 | PIERRE, Danaelle | Hardee |
18.78 | Avon Park Red Devil Invitational |
| 4 | JOHNSON, Mahagony | Avon Park |
16.41 | Avon Park Red Devil Invitational |
| 5 | WATT, Kamila | Avon Park |
17.77 | Bob English Invitational 2026 |
| 6 | DESTINO , Zarmiah | Avon Park |
20.36 | Tenoroc High School Invitational at SEU |
| 7 | TOWNER, Riley | Lake Placid |
21.57 | Avon Park Invitational |