| Lane | Athlete | Team | Note | 
|---|---|---|---|
| 3 | VANN, Trinadee | Fort Pierce Westwood | |
| 4 | MELANDES, Kammile | Fort Pierce Central | |
| 5 | DEFLORIO, Alana | John Carroll | 
| Lane | Athlete | Team | Note | 
|---|---|---|---|
| 1 | THOMPKINS, Debrionna | Fort Pierce Westwood | |
| 2 | HOGAN, Tianna | Lincoln Park | 28.38 Bonita Williams Invitational | 
| 3 | CLAYTON, Brianna | Fort Pierce Central | Astronaut Preseason Classic | 
| 4 | CLARK, Amber | Lincoln Park | FHSAA 2A District 13 | 
| 5 | VEREEN, Kiana | Fort Pierce Central | Astronaut Preseason Classic | 
| 6 | RIVERA-SEARS, Geliya | John Carroll | FHSAA 1A District 11 | 
| 7 | BEAUSEJOUR-JOSEPH, Yasmine | Lincoln Park | |
| 8 | STEWART, Raziyah | Fort Pierce Westwood |