| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Legacy High School | A | Desir | Taylor | Townsley | Bernard | DNS | Jensen Beach Meet #2 |
| 3 | Martin County |
A | Osdon | Lindsay | Bussom | Hopkins | 4:27.01 | Benjamin Invite #1 2026 (approved Qualifier) |
| 4 | South Fork |
A | Rodgers | Weick | Robinson | Brinn | 4:10.62 | Kings Academy Varsity 2 Sanctioned Meet |
| 5 | Jupiter |
A | Hanssen | Sandel | Roberts | Maggio | 4:16.58 | Dr. Garcia Meet #2 |
| 6 | West Boca Raton |
A | Gonzales | Julien-Jeune | Whitfield | Otero | DNS | Monsignior Pace 2 |
| 7 | Fort Pierce Central |
A | Spence | Veal | Tommie | Wilder | NT |