| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Legacy High School | A | Rodriguez | Gooden | Haynes | Williams | 46.23 | Cobra Relays 2026 |
| 2 | Martin County |
A | Montisol | Campbell | Dennis | Oh | 44.95 | Jensen Beach Invitational 2026 |
| 3 | South Fork |
A | Bailey | Miguel | Pachowicz | Valenti | 44.41 | Kings Academy Varsity 2 Sanctioned Meet |
| 4 | West Boca Raton |
A | Duggan | St. Pierre | Santacruz | Wooten | DNS | Monsignior Pace 2 |
| 5 | Fort Pierce Central |
A | Dixon | Ransfer | Nathan-Marsh Jr | Victorin | 44.06 | Cobra Relays 2026 |
| 6 | Tradition Prep | A | Monaco | Joseph | Lopez | Chevres | 44.81 | Vero Beach HS Relay Championships (FHSAA Sactioned) |
| 7 | Jupiter |
A | Peters | Garabrant | Quinn | Rebimbas | 46.19 | John I Leonard Invitational 1 |