| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clewiston |
A | Magana | Graham | Love | Coring | 4:05.11 | Jensen Beach Invitational 2026 |
| 2 | Martin County |
A | Dennis | St. Juste | McLean | Allison | 3:45.27 | Benjamin Invite #1 2026 (approved Qualifier) |
| 3 | South Fork |
A | Christoff | Harris | Quintero | Valenti | 3:29.86 | Kings Academy Varsity 2 Sanctioned Meet |
| 4 | West Boca Raton |
A | Thomas Jr. | Haynes | Duggan | St. Pierre | DNS | Jeff Fossen Memorial Invite 2026 |
| 5 | Fort Pierce Central |
A | Lamarre | Brown | Gilchrist | Baker | 3:29.84 | Cobra Relays 2026 |
| 6 | Jupiter |
A | Burke | Click | Dettman | Baer | 3:36.64 | John I Leonard Invitational 1 |
| 7 | Legacy High School | A | Mimnaugh | Scalise | Meant | Garcia | 3:59.71 | Jensen Beach Meet #2 |