| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | South Fork |
A | 9:53.95 | Cobra Relays 2025 |
| 2 | Jupiter |
A | 10:24.25 | Dr. Garcia Invite #2 2025 |
| 3 | Treasure Coast | A | 10:46.12 | Cobra Relays 2025 |
| 4 | Fort Pierce Central |
A | 11:43.18 | Cobra Relays 2025 |
| 5 | Lincoln Park |
A | 11:55.46 | SLC Championships 2025 |
| 6 | Martin County |
A | NT |