| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Martin County |
A | 8:36.80 | Cobra Relays 2025 |
| 2 | Treasure Coast | A | 8:39.60 | Park Vista #1 2025 |
| 3 | Jupiter |
A | 8:43.77 | Dr. Garcia Invite #2 2025 |
| 4 | Fort Pierce Central |
A | 8:58.90 | Cobra Relays 2025 |
| 5 | Port St. Lucie |
A | 9:18.05 | SLC Championships 2025 |
| 6 | South Fork |
A | 9:26.40 | Cobra Relays 2025 |
| 7 | Tradition Prep | A | 10:06.36 | Jensen Beach Invite 2025 |
| 8 | Pine School |
A | 10:19.40 | Jensen Beach Invite 2025 |
| 9 | Lincoln Park |
A | NT |