| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | HAMILTON, Shantell | Tradition Prep | NT | NT |
| 4 | GOMEZ, Olivia | Lincoln Park |
21.06 | SLC Championships 2025 |
| 5 | GRAHAM, Skye | Fort Pierce Central |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | EDWARDS, Nyelie | Martin County |
20.65 | Cobra Relays 2025 |
| 2 | PHILLIPPE, Tylah | Tradition Prep | 20.44 | SLC Championships 2025 |
| 3 | MOODY, Jamaria | Fort Pierce Westwood |
19.90 | SLC Championships 2025 |
| 4 | MCCAIN, Skyler | Jupiter |
16.93 | Benjamin Invite #2 2025 (Sanctioned) |
| 5 | FEQUIERE, Jada | Fort Pierce Central |
19.41 | Cobra Relays 2025 |
| 6 | LANE, Raniyah | Fort Pierce Central |
20.03 | Cobra Relays 2025 |
| 7 | MUNNINGS, Deondra | Fort Pierce Central |
20.47 | SLC Championships 2025 |
| 8 | BACON, Ra'Mya | Lincoln Park |
20.72 | SLC Championships 2025 |