| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Jensen Beach |
A | Chrysler | Webley | Blackshell | Fell | 45.59 | |
| 2 | Martin County |
A | Montisol | Campbell | Gilbride | Oh | 44.95 | Jensen Beach Invitational 2026 |
| 3 | Fort Pierce Westwood |
A | Ford | Greer | Lynch | Martin | 44.04 | Cobra Relays 2025 |
| 4 | John Carroll |
A | Pittman | Coleman | Summerall | RICHARDSON | 43.67 | Jensen Beach Invitational 2026 |
| 5 | Tradition Prep | A | Monaco | Heredia | Lopez | Chevres | 43.87 | Bulldog Invitational 2025 |
| 6 | Sebastian River |
A | Marshall | Gibbs | St. John | Sears | 44.15 | FHSAA 3A Region 4 |
| 7 | Somerset College Prep | A | Alperis | Cabrera | Horne | Elipha | 45.45 | |
| 8 | Legacy High School | A | Thompson | Serrano | Rodriguez | Williams | DNF | Vero Beach HS 2026 Preseason Classic |